Drosophila promoters

These vectors have the Ubiquitin promoter with optional N-terminal epitope tags following the initial ATG. We have used this promoter with fusions to cDNAs of Mad to rescue maternal and zygotic phenotypes (see Sekelsky et al. 1995) and mei-9 to rescue phenotypes in somatic cells and female meiosis (see Yildiz et al. 2004). The pBU series are in pBlueScript KS+; the pWU vectors are in our pP{70w} P element transformation vector.

pBUF

FLAG epitope (or no tag)

Map

Sequence

GCK2 file

pBUHA

HA epitope

Map

Sequence

GCK2 file

pBUSH

Six histidine tag

Map

Sequence

GCK2 file

pWUM6

Six Myc epitopes

Map

Sequence

GCK2 file

pWUM6b

Six Myc epitopes

Map

Sequence

GCK2 file

Drosophila P element transformation vectors

pP{70wi}

Small; many cloning sites

Map

Sequence

GCK2 file

pP{BeloBAC}

BAC vector for cloning repeats

Map

Sequence

GCK2 file

Drosophila gene targeting vectors

See also our Guide to Gene Targeting

References reporting successful targeting with these vectors.

The pP{Target} series vectors are for use in the ends-in targeted gene replacement method of Rong and Golic. Each has a polylinker, white marker gene, and I-CreI site, flanked by FRT sites and P element ends.

pP{Target}

mini-white from CaSpeR

Map

Sequence

GCK2 file

pP{TargetB}

Smaller version of above

Map

Sequence

GCK2 file

pP{Target70}

hsp70::white cDNA

Map

Sequence

GCK2 file

pP{Target70i}

hsp70::white cDNA, with intron

Map

Sequence

GCK2 file

Rong and Golic used "ends-in" (insertional) targeting. For some purposes, "ends-out" (gene replacement) is preferable. Ends-out targeting has recently been shown to work in Drosophila (Gong and Golic, unpublished). Our vector for this method is pP{EndsOut}. A genomic region is cloned into the polylinker, and the gene of interest is disrupted or replaced with the hsp70::white cDNA (which also serves as a marker for transformation).

pP{EndsOut2}

For ends-out gene replacement

Map

Sequence

GCK2 file

pBS-70w

hsp70-white cDNA

Map

Sequence

GCK2 file

pP{whiteOut2}

For ends-out using white for transformation,
but not for selecting targeted events

Map

Sequence

GCK2 file