The Sekelsky Lab

pWUM6

pWUM6 has a 1.7-kb Ubiquitin promoter, which drives a moderate level of expression in all tissues; heat-shock causes about 3X increased transcription (Lee et al., MCB 8:4727, 1988). The ATG corresponding to the initial codon is followed by sequences encoding six copies of the Myc epitope. Coding sequences can be cloned in-frame using the sites in the polylinker. The module is in pP{70w} P element transformation vector. pWUM6b is inserted into this vector in the opposite orientation, so it has different cloning sites.

pWUM6 Features

pWUM6 Sequence

CATGATGAAATAACATAAGGTGGTCCCGTCGGCAAGAGACATCCACTTAACGTATGCTT GCAATAAGTGCGAGTGAAAGGAATAGTATTCTGAGTGTCGTATTGAGTCTGAGTGAGAC AGCGATATGATTGTTGATTAACCCTTAGCATGTCCGTGGGGTTTGAATTAACTCATAAT ATTAATTAGACGAAATTATTTTTAAAGTTTTATTTTTAATAATTTGCGAGTACGCAAAg ctagctatagttctagaggtaccgggcccccctcgaggtcgacgcgcagatcgccgatg ggcgtggcgccggagggcaccttcaccttgccgtacggcttgctgttcttcgcgttcaa aatctccagctccattttgctttcggtgcgcttgcaatcagtactgtccaaaatcgaaa atcgccgaaccgtagtgtgaccgtgcggggctctgcgaaaataaacttttttaggtata tggccacacacggggaaagcacagtggattatatgttttaatattataatatgcaggtt ttcattacttatccagatgtaagcccacttaaagcgatttaacaattatttgccgaaag agtataaacaaatttcacttaaaaatggattaagaaaagcttgtgtaagattatgcgca gcgttgccagatagctccatttaaaacacttcaaaaacaataagttttgaaaatatata cataaatagcagtcgttgccgcaacgctcaacacatcacacttttaaaacaccctttac ctacacagaattactttttaaatttccagtcaagctgcgagtttcaaaattatagccgg tagagaagacagtgctatttcaaaagcaaactaacaagggtcttaaattccaaaacacc aatcctaacaagccttggacttttgtaagtttagatcaaaggtggcattgcattcaatg tcatggtaagaagtaggtcgtctaggtagaaatcctcattcagccggtcaagtcagtac gagaaaggtctcaatttgaaattgtcttaaaaatattttattgttttgtactgtggtga gtttaaacgaaaaacacaaaaaaaaagtgatacacagaaatcataaaaaattttaatac aaggtattcgtacgtatcaaaaacatttcggcacaattttttttctctgtactaaagtg ttacgaacactacggtattttttagtgattttcaacggacaccgaaggtatataaacag cgttcgcgaacggtcgcctTCAAAACCAATTGACATTTGCAGCAGCAAGTACAAGCAGA AAGTAAAGCGCAATCAGCGAAAAATTTATACTTAATTGTTGGTGATTAAAGTACAATTA AAAGAACATTCTCGAAAGTCACAAGAAACGTAAgtttttaactcgctgttaccaattag taataagagcaacaagacgttgagtaatttcaagaaaaactgcatttcaaggtctttgt tcggccattttttttttattcaacgctctacgtaattacaaaataagaaattggcagcc acgcatcttgttttcccaatcaattggcatcaaaacgcaaacaaatctataaataaaac ttgcgtgttgattttcgccaagatttattggcaaattgtgaaattcgcagtgacgcatt tgaaaattcgagaaatcacgaacgcactcgagcatttgtgtgcatgttattagttagtt agttctttgcttaattgaagtattttaccaacgaaatccacttatttttagctgaaata gagtaggttgcttgaaacgaaagccacgtctggaaaatttcttattgcttagtagttgt gacgtcaccatatacacacaaaataatgtgtatgcatgcgtttcagctgtgtatatata catgcacacactcgcattatgaaaacgatgacgagcaacggaacaggtttctcaactac ctttgttcctgtttcttcgctttcctttgttccaatattcgtagagggttaataggggt ttctcaacaaagttggcgtcgataaataagtttcccatttttattccccagccaggaag ttagtttcaatagttttgtaatttcaacgaaactcatttgatttcgtactaattttcca catctctattttgacccgcagAATAATCCACCATGGGGCAGGGATCCCATCGATTTAAA GCTATGGAGCAAAAGCTCATTTCTGAAGAGGACTTGAATGAAATGGAGCAAAAGCTCAT TTCTGAAGAGGACTTGAATGAAATGGAGCAAAAGCTCATTTCTGAAGAGGACTTGAATG AAATGGAGCAAAAGCTCATTTCTGAAGAGGACTTGAATGAAATGGAGCAAAAGCTCATT TCTGAAGAGGACTTGAATGAAATGGAGAGCTTGGGCGACCTCACCATGGAGCAAAAGCT CATTTCTGAAGAGGACTTGAATTCAAGGCCTCTCGAGCCTCTAGAGCGGCCGCCTGCAG CTGGCGCCATCGATACGCGTACGTCGCGACCGCGGacatgtacagagctcgagtttttt tttttttttttttttttttttattggcacaaatataattacattagctttttgaggggg caataaacagtaaacacgatggtaataatggtaaaaaaaaaaaacaagcagttatttcg gatatatgtcggCTACTCCTTGCGTCGGGCCCGAAGTCTTAGAGCCAGATATGCGAGCA CCCGGAAGCTCACGATGAGAATGGCCAGACCCACGTAGTCCAGCGGCAGATCGGCGGCG GAGAAGTTAAGCGTCTCCAGGATGACCTTGCCCGAACTGGGGCACGTGGTGTTCGACGA TGTGCAGCTAATTTCGCCCGGCTCCACGTCCGCCCATTGGTTAATCAGCAGACCCTCGT TGGCGTAACGGAACCATGAGAGGTACGACAACCATTTGAGGTATACTGGCACCGAGCCC GAGTTCAAGAAGAAGCCGCCAAAGAGCAGGAATGGTATGATAACCGGCGGACCCACAGA CAGCGCCATCGAGGTCGAGGAGCTGGCGCAGGATATTAGATATCCGAAGGACGTTGACA CATTGGCCACCAGAGTGACCAGCGCCAGGCAGTTGAAGAAGTGCAGCACTCCGGCCCGC AGTCCGATCATCGGATAGGCAATCGCCGTGAAGACCAGTGGCACTGTGAGAAAAAGCGG CAATTCGGCAATCGTTTTGCCCAGAAAGTATGTGTCACAGCGATAAAGTCGACTTCGGG CCTCCCTCATAAAAACTGGCAGCTCTGAGGTGAACACATTTATCGTGGCAAAGACGTTT TGAAAGGTCATGTTGGTCAGGAAGAGGAAGATGGCTCCGTTGATATTCATCACACCCAC TTGCGTGAGTTGTTGGCCCAAAAAGATGAGGCCAATCAAGATGGCAACCATCTGCAAAT TAAAATGTTACTCGCATCTCATTAATATTCGCGAGTTAAATGAAATTTATTTATCTTCG TTGTCTGAATAAGTCGCACTTTTACGAGGAGTGGTTCCTTGAGCACCGACAGCCAGGAT CGCCACAGGACCGCCCGGAACTGCATGAACCAGGTGGCCTTGTAGGTGTACCCATTCTC CGGCTGCTCCAGTGGCTTCTCCAGATTTTTGGTGGCCAACAACTGCTCCATATCCCGGG CTACTTTGCTAATGGCAAAATTGTCGCCATATCTTGGCGATCCGATCACGGGACTCGAT CTCCCGTCCGGGCACAACGGCCAACACCTGTACGTAAAAGTCCGCCGGATTGTAGTTGG TAGGACACTGGGCACCCACGTAGGAAAAGAAGTCGACGGCTTCGCTGGGAGTGCCCAAG AAAGCTACCCTGCCCTCGGCCATCAGAAGGATCTTGTCAAAGAGCTCAAACAGCTCGGA AGACGGCTGATGAATGGTCAGGATGACGGTCTTGCCCTTCTGCGACAGCTTCTTCAGCA CCTGGACGACGCTGTGGGCGGTAAATGAGTCCAGTCCGGAGGTGGGCTCATCGCAGATC AGAAGCGGCGGATCGGTTAGTGCCTCGGAGGCGAATGCCAGACGCTTCCTTTCTCCGCC GGACAGACCTTTCACCCTGCCGGGCACACCGATGATCGTGTGCTGACATTTGCTGAGCG AAAGCTCCTGGATCACCTGATCCACGCGGGCCACTCGCTGCCGATAGGTCAGATGTCGT GGCATCCGCACCATGGCTTGGAAAATCAGGTGTTCCCTGGCCGTTAGGGAGCCGATAAA GAGGTCATCCTGCTGGACATAGGCGCACCTGGCCTGCATCTCCTTGGCGTCCACAGGTT GGCCATTGAGCAGTCGCATCCCGGATGGCGATACTTGGATGCCCTGCGGCGATCGAAAG GCAAGGGCATTCAGCAGGGTCGTCTTTCCGGCACCGGAACTGCCCATCACGGCCAAAAG TTCGCCCGGATAGGCCACGCCGCAAACGTTCTTGAGCAAATGTTTCCTGGGCGCCGGTA TGTGTCGCTCGTTGCAGAATAGTCCGCGTGTCCGGTTGACCAGCTGCCGCCATCCGGAG CCCGGCTGATTGACCGCCCCAAAGATGTCCATATTGTGCCAGGCATAGGTGAGGTTCTC GGCTAGTTGGCCGCTCCCTGAACCGGAGTCCTCCGGCGGACTGGGTGGCAGGAGCGTGC CGTAGTTTTTGGCCTGCCCGAAGCCCTGGTTAATGCAGCTCTGCGAAGCGTCCGCTGTC ACCCTGCAATGATAGGGGATCTCAAATATCAAATTGTTCAGATGCTCGGCAGATGGGTG TTTGCTGCCTCCGCGAATTAATAGCTCCTGATCCTCTTGGCCCATtgccgggatttttc acactttcccctgcttacccacccaaaaccaatcacccacccaatcactcaaaaaacaa acaaaaataagaagcgagaggagttttggcacagcactttgtgtttaattgatggcgta aaccgcttggagcttcgtcacgaaacctcgtgccgaattgacgctatcgacctattcAG AGTTCTCTTCTTGTCTTCAATAATGACTTCTTGGTTGATTTCAGTAGTTGCAGTTTTTA GTTTAATTACTTGGTTGTTGGTTACTTTTAATTGATTCACTTTAACTTGCACTTTATTG CAGATTGTTTAGCTTGTTCGCTGCGCTTGTTTGTTTGCTCAGCTTGCTTCGCGATGTGT TCACTTTGcttgtttgaattgaattgacgctccgtcgacgaagcgcctctatttatact ccggcgctcttttcgcgaacattcgaggcgcgctctctcgaagcaacgagaatagagtg ccgtttactgtgcgacagagtgagagagcaatagtacagagagggagagtcacaaaacg aatagagaataacggggggccggccactagcggctgctgctctAAACGACGCATTTCGT ACTCCAAAGTACGAATTTTTTCCCTCAAGCTCTTATTTTCATTAAACAATGAACAGGAC CTAACGCACAGTCACGTTATTGTTTACATAAATGATTTTTTTTACTATTCAAACTTACT CTGTTTGTGTACTCCCACTGGTATAGCCTTCTTTTATCTTTTCTGGTTCAGGCTCTATC ACTTTACTAGGTACGGCATCTGCGTTGAGTCGCCTCCTTTTAAATGTCTGACCTTTTGC AGGTGCAGCCTTCCACTGCGAATCATTAAAGTGGGTATCACAAATTTGGGAGTTTTCAC CAAGGCTGCACCCAAGGCTCTGCTCCCACAATTTTCTCTTAATAGCACACTTCGGCACG TGAATTAATTTTACTCCAGTCACAGCTTTGCAGCAAAATTTGCAATATTTCATTTTTTT TTATTCCACGTAAGGGTTAATGTTTTCAAAAAAAAATTCGTCCGCACACAACCTTTCCT CTCAACAAGCAAACGTGCACTGAATTTAAGTGTATACTTCGGTAAGCTTCGGCTTTCGA CGGGACCACCTTATGTTATTTCATCATGGGCCAGACCCACGTAGTCCAGCGGCAGATCG GCGGCGGAGAAGTTAAGCGTCTCCAGGATGACCTTGCCCGAACTGGGGCACGTGGTGTT CGACGATGTGCAGCTAATTTCGCCCGGCTCCACGTCCGCCCATTGGTTAATCAGCAGAC CCTCGTTGGCGTAACGGAACCATGAGAGGTACGACAACCATTTGAGGTATACTGGCACC GAGCCCGAGTTCAAGAAGAAGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCA CAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGA TACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAG GTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGA CACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGT AGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAG TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCT TGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGAT TACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATC TTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGA GTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCT GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGG GAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGC TCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTG CAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGT TCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACG CTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACAT GATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGA AGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTAC TGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCT GAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACC GCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAA ACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCA ACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGG CAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTT CCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATAT TTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG CCACCTGCGAAGTCTTAGAGCCAGATATGCGAGCACCCGGAAGCTCACGATGAGAATGG CCAGAC

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