The Sekelsky Lab

pBUSH

pBUSH has a 2-kb Ubiquitin promoter, which drives a moderate level of expression in all tissues; heat-shock causes about 3X increased transcription (Lee et al., MCB 8:4727, 1988). The ATG corresponding to the initial codon is followed by sequences encoding the six histidine residues. Coding sequences can be cloned in-frame using the sites in the polylinker. To use this promoter construct without a tag, use pBUF.

pBUSH Sequence

GGTACCGGGCCCCCCCTCGAGGTCGACTCTAGAGGATCTTGTCGCCGGAACGCAGCGACAGAGA TTCCAATGTGTCCGTATCTTTCAGGCTTTTGCCCTTCAGTTCCAGACGAAGCGACTGGCGATTC GCGTGTGGGGTCTGCTTCAGGGTCTTGTGAATTAGGGCGCGCAGATCGCCGATGGGCGTGGCGC CGGAGGGCACCTTCACCTTGCCGTACGGCTTGCTGTTCTTCGCGTTCAAAATCTCCAGCTCCAT TTTGCTTTCGGTGCGCTTGCAATCAGTACTGTCCAAAATCGAAAATCGCCGAACCGTAGTGTGA CCGTGCGGGGCTCTGCGAAAATAAACTTTTTTAGGTATATGGCCACACACGGGGAAAGCACAGT GGATTATATGTTTTAATATTATAATATGCAGGTTTTCATTACTTATCCAGATGTAAGCCCACTT AAAGCGATTTAACAATTATTTGCCGAAAGAGTATAAACAAATTTCACTTAAAAATGGATTAAGA AAAGCTTGTGTAAGATTATGCGCAGCGTTGCCAGATAGCTCCATTTAAAACACTTCAAAAACAA TAAGTTTTGAAAATATATACATAAATAGCAGTCGTTGCCGCAACGCTCAACACATCACACTTTT AAAACACCCTTTACCTACACAGAATTACTTTTTAAATTTCCAGTCAAGCTGCGAGTTTCAAAAT TATAGCCGGTAGAGAAGACAGTGCTATTTCAAAAGCAAACTAACAAGGGTCTTAAATTCCAAAA CACCAATCCTAACAAGCCTTGGACTTTTGTAAGTTTAGATCAAAGGTGGCATTGCATTCAATGT CATGGTAAGAAGTAGGTCGTCTAGGTAGAAATCCTCATTCAGCCGGTCAAGTCAGTACGAGAAA GGTCTCAATTTGAAATTGTCTTAAAAATATTTTATTGTTTTGTACTGTGGTGAGTTTAAACGAA AAACACAAAAAAAAAGTGATACACAGAAATCATAAAAAATTTTAATACAAGGTATTCGTACGTA TCAAAAACATTTCGGCACAATTTTTTTTCTCTGTACTAAAGTGTTACGAACACTACGGTATTTT TTAGTGATTTTCAACGGACACCGAAGGTATATAAACAGCGTTCGCGAACGGTCGCCTTCAAAAC CAATTGACATTTGCAGCAGCAAGTACAAGCAGAAAGTAAAGCGCAATCAGCGAAAAATTTATAC TTAATTGTTGGTGATTAAAGTACAATTAAAAGAACATTCTCGAAAGTCACAAGAAACGTAAGTT TTTAACTCGCTGTTACCAATTAGTAATAAGAGCAACAAGACGTTGAGTAATTTCAAGAAAAACT GCATTTCAAGGTCTTTGTTCGGCCATTTTTTTTTTATTCAACGCTCTACGTAATTACAAAATAA GAAATTGGCAGCCACGCATCTTGTTTTCCCAATCAATTGGCATCAAAACGCAAACAAATCTATA AATAAAACTTGCGTGTTGATTTTCGCCAAGATTTATTGGCAAATTGTGAAATTCGCAGTGACGC ATTTGAAAATTCGAGAAATCACGAACGCACTCGAGCATTTGTGTGCATGTTATTAGTTAGTTAG TTCTTTGCTTAATTGAAGTATTTTACCAACGAAATCCACTTATTTTTAGCTGAAATAGAGTAGG TTGCTTGAAACGAAAGCCACGTCTGGAAAATTTCTTATTGCTTAGTAGTTGTGACGTCACCATA TACACACAAAATAATGTGTATGCATGCGTTTCAGCTGTGTATATATACATGCACACACTCGCAT TATGAAAACGATGACGAGCAACGGAACAGGTTTCTCAACTACCTTTGTTCCTGTTTCTTCGCTT TCCTTTGTTCCAATATTCGTAGAGGGTTAATAGGGGTTTCTCAACAAAGTTGGCGTCGATAAAT AAGTTTCCCATTTTTATTCCCCAGCCAGGAAGTTAGTTTCAATAGTTTTGTAATTTCAACGAAA CTCATTTGATTTCGTACTAATTTTCCACATCTCTATTTTGACCCGCAGAATAATCCACCATGCG CGGCTTCCATCACCATCACCATCACAAGGCCTCATCGATAAGCTTGATATCGAATTCCTGCAGC CCGGGCAGGGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAATTCGCCCTATA GTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGT TACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCC CGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCGACGCGCCCTGTA GCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGC CCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGT CAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCA AAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCC TTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAAC CCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAA ATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCCCA GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAA ATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAG TATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGG GTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTT TCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCA CAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAG TGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTT TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCA TACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATT AACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAG CCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTAT CGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG ATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGA TTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCAT GACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAA GGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGC TACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTT CAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAG AACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTG GCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTC GGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGA TACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATC CGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTA TCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCA GGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCT GGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGC CTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAG GAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCA GCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTA GCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATT GTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCGGAATTA ACCCTCACTAAAGGGAACAAAAGCTG

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