These electropherograms were produced by an ABI Prism 377 automated DNA sequencer. The trace of the top is from wild-type; the trace on the bottom is from mei-9a, the first mutant allele recovered by Baker and Carpenter. The amino acid sequence encoded by this region is given, with the alteration responsible for the mei-9 mutant phenotype indicated. A G-to-A transition predicts a change of aspartic acid to aparagine. This particular Asp is conserved in all homologues of MEI-9, and has been suggested by Aravind to be involved in catalysis.
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© Newtron Laboratories
