Graduate Student, Curriculum in Genetics and Molecular Biology
I am interested in how structure-selective endonucleases generate meiotic and mitotic crossovers. I began by exploring the functions of the putative Holliday junction resolvase GEN in Drosophila melanogaster. Its orthologs, yeast Yen1 and human GEN1, play a secondary role to Mus81 during DNA damage repair and meiotic recombination. However, Drosophila Gen mutants display much more severe phenotypes than mus81 mutants, suggesting that GEN plays a more primary role in the response to DNA damage. My research has expanded to include all of the known Holliday junction resolvases. I am using both genetic and biochemical approaches to explore the role of these endonucleases in vivo an in vitro. This work will help identify specific roles for structure-selective endonucleases in double-strand break repair and replication fork restart.
Publications from research in the Sekelsky Lab
Talks at conferences
Curriculum in Genetics and Molecular Biology training grant, 2009-2010